The present manuscript examines the signaling pathways underlying the apoptotic activity of capsaicin in human small cell lung cancer sclc in vitro and in vivo. A following treatment with three concentrations of capsaicin for 24 h, the cells were incubated with fitcconjugated annexin vpi and. Sep 03, 2010 capsaicin has been shown to induce apoptosis cell death in cancer cells. Studies have shown that capsaicin induces apoptosis in colon cancer cells through generation of ros, disruption of mitochondrial membrane potential and via caspase3 dependent and p arp. Dihydrocapsaicin dhc, a saturated structural analog of. In vitro and in vivo induction of apoptosis by capsaicin in pancreatic cancer cells is mediated through ros generation and mitochondrial death pathway. Studies in neuronal cells show that capsaicin exerts its biological activity via. A following treatment with three concentrations of capsaicin for 24 h, the cells were incubated. Although capsaicin, a pungent component of red pepper, is known to induce apoptosis in several types of cancer cells, the mechanisms underlying capsaicininduced cytotoxicity are unclear. In addition to the caspase3 activation, capsaicin also induced cytochrome c release and decrease in bcl2 protein expression with no changes in the level of bax. It is also a treasure trove of healing for the body. Crossrefpubmed zhang r, humphreys i, sahu rp, shi y, srivastava sk.
Anticancer properties of capsaicin against human cancer. Oct 12, 2016 renal cell carcinoma rcc is one of the tumors most refractory to chemotherapy to date. Super cancerkiller capsicum and green tea by now, the benefits of drinking green tea are well known to most healthconscious people. In contrast, capsaicin has also been considered to promote the growth of cancer cells.
Pdf capsaicin induces apoptosis and autophagy in human. Capsaicin induces autophagy and apoptosis in human. Capsaicin effectively inhibited tumor growth and induced apoptosis in nonobese diabetic severe combined immunodeficient mice and, even when administered intraperitoneally i. The ability of capsaicin to inhibit the proliferation of a panel of erpositive and. Recently, capsaicin has been found to inhibit the growth of various immortalized and malignant cells and induces apoptosis in transformed cells. But did you know that capsaicin can actually create apoptosis, or cancer cell death. Capsaicin and small cell lung cancer therapy division of. Capsaicin induced apoptosis was in association with the elevation of intracellular reactive oxygen species production. Capsaicin is a vanilloid quinone analog that inhibits the plasma membrane electron transport pmor system and induces apoptosis in transformed cells. The antiproliferative and apoptotic effects of capsaicin.
Jan 12, 2018 capsaicin induced trib3 upregulation promotes apoptosis in cancer cells rongjaan lin,1,2 ijung wu,1,2 joyu hong,3 banghung liu,4 rueiyue liang,4 teinming yuan,5 showmei chuang4,6,7 1college of life sciences, national chung hsing university, 2taichung hospital, ministry of health and welfare, 3bachelor program of biotechnology, college of agriculture and natural resources, 4institute of. It is an irritant for mammals, including humans, and produces a sensation of burning in any tissue with which it comes into contact. Recent studies focusing on the chemopreventive and chemotherapeutic effects of capsaicin have demonstrated that it can decrease growth in various human cancer cell systems eg, liver, lung, colon, pancreas, stomach, prostate, leukocyte, breast, esophagus, and tongue through its effects on cell cycle arrest and apoptosis. One study in 2010 cancer research by researchers at the hormel institute, university of minnesota, linked capsaicin to a higher risk of skin cancer, although the molecular mechanisms. Capsaicininduced apoptosis and reduced release of reactive. Trpv6 mediates capsaicininduced apoptosis in gastric cancer. Our results from the studies funded by the parent r15 grant showed that capsaicin the spicy ingredient of chili peppers displays potent apoptotic activity in sclc in cell. M camptothecin showed significantly greater apoptosis than 10. Capsaicin is the major pungent ingredient in red peppers. Capsaicin induces apoptosis and modulates mapk signaling in. Effect of capsaicin on proliferation, cell cycle and apoptosis of breast cancer cells. Studies in neuronal cells show that capsaicin exerts its biological activity via the.
Capsicum, cayenne, chilli pepper and cancer canceractive. However, research findings have also shown that it can also act as a carcinogen, especially at the tumor promotion stage. Next, we studied capsaicins activity on cell cycle and apoptosis. I have written about the vast benefits of green tea on breast cancer as well as for general health on this blog in the past.
Capsaicin induces cell cycle arrest and apoptosis in human kb. Capsaicin 8methylnvanillyl6nonenamide is an active component of chili peppers, which are plants belonging to the genus capsicum. Capsaicin synergizes with camptothecin to induce increased. Abnormal regulation of apoptosis is an important aspect of tumour development. Involvement of endoplasmic reticulum stress in capsaicin. Notably, capsaicin blocked breast cancer cell migration in vitro and decreased by 50% the size of mdamb231 breast cancer tumors growing. Caspase3 activity assays revealed that capsaicin caused programmed cell death in h69 human sclc cells at 36 hours. Capsaicin, an extract of red chilli peppers, has been shown to inhibit growth of melanoma and other malignant cell lines and ha141 is an organic compound that directly induces apoptosis by binding to bcl2 protein.
Capsaicin has been shown to induce apoptosis cell death in cancer cells. Capsaicininduced apoptosis of fadu human pharyngeal squamous. Selective induction of apoptosis by capsaicin in transformed. Keywords capsaicin chili peppers cancer apoptosis stress signal. Capsaicin induces the apoptosis of cancers cells, including myeloid leukemia and. Aspc1 cells were pretreated with 20 lm sp600125 for 1 h and then exposed to 150 lm capsaicin for 24 h in the presence of inhibitor. Here, we showed that dihydrocapsaicin dhc, an analog of capsaicin, is a potential inducer of autophagy. The antiproliferative effects of capsaicin on human kb cancer cells are. In this study, we investigated the apoptotic effect of capsaicin on human pancreatic cancer cells in both in vitro and in vivo systems, as well as the possible mechanisms involved. Capsaicin induces apoptosis and autophagy in human. Capsaicin is known to have tumor suppressive effects. Nyas publications the new york academy of sciences. Capsaicin, the pungent ingredient of chili peppers, displays potent antineoplastic activity in a wide array of human cancer cells. Research paper capsaicin mediates cell cycle arrest and.
Capsaicininduced trib3 upregulation promotes apoptosis in. Capsaicininduced trib3 upregulation promotes apoptosis in cancer cells rong jaan lin,1,2 ijung wu,1,2 joyu hong,3 banghung liu,4. Each sample was measured in duplicate and the entire experiment was repeated three independent times. May 31, 2014 capsaicin, the pungent ingredient of chili peppers, displays potent antineoplastic activity in a wide array of human cancer cells. The benefits of capsaicin are clear, and we hope that in the future it may be able to harnessed in a wonderful way to help to defeat cancer. Pdf role of capsaicin in cancer prevention researchgate. Effects of capsaicin on induction of apoptosis and inhibition. Our results from the studies funded by the parent r15 grant showed that capsaicin the spicy ingredient of chili peppers displays potent apoptotic activity in sclc in cell culture, in chicken. Capsaicin induces apoptosis in human gastric cancer cells. Gastric cancer gastric cancer is a malignancy that occurs from the lining of the stomach. Trpv6 mediates capsaicininduced apoptosis in gastric cancer cells. Capsaicininduced apoptosis was revealed by the presence of. Capsaicintreated breast cancer cells showed also decreased levels of cyclin d1, increased activity of p27 kip1 and caspase3, 7, 9 as well as appearance of the parp cleavage product, which was. In this article, we have investigated the effects of capsaicin on apoptosis in relation to ampk activation in colon cancer cell.
Here, we reported that capsaicin had a profound anti proliferative effect on human colon cancer cells via inducing cell cycle g0g1 phase arrest and apoptosis, which was associated with an increase of p21, bax and cleaved parp. Therefore, the current study investigated capsaicininduced apoptosis and autophagy in npctw01 cells. Capsaicin induces apoptosis and autophagy in human melanoma cells. Although capsaicin, a pungent component of red pepper, is known to induce apoptosis in several types of cancer cells, the mechanisms underlying capsaicin induced cytotoxicity are unclear. Capsaicin, a pungent phytochemical in a variety of red peppers of the genus capsicum, has shown an antiproliferative effect on various human cancer cell lines. Oct 04, 2018 recent studies focusing on the chemopreventive and chemotherapeutic effects of capsaicin have demonstrated that it can decrease growth in various human cancer cell systems eg, liver, lung, colon, pancreas, stomach, prostate, leukocyte, breast, esophagus, and tongue through its effects on cell cycle arrest and apoptosis. Capsaicin, a spicy component of hot pepper, induces. Capsaicin, main pungent ingredient of hot chilli peppers, has been shown to have anticarcinogenic effect on various cancer cells through multiple mechanisms.
Our results showed that capsaicin induces apoptosis in both cells, although cancer cells are more susceptible. Capsaicin causes greater apoptosis in human sclc cell lines than in normal lung cells. The benefits of capsaicin and its help against cancer. In the present study, we investigated the effects of capsaicin on the induction of preadipocytic apoptosis and inhibition of adipocytic triglyceride content. Capsaicin induced apoptosis was revealed by the presence of. Studies in neuronal cells show that capsaicin exerts its biological activity via the transient. Our study showed that the capsaicin treatment significantly induced cell apoptosis and led to prominent upregulation of apoptosis related proteins. The antiproliferative effects of capsaicin on human kb cancer cells are still unknown. Pdf in vitro and in vivo induction of apoptosis by. M for 24 hrs, no obvious cell cycle arrest was observed. Pepper component hot enough to trigger suicide in prostate. The results may expand our understanding of the apoptosis and autophagyrelevant signaling pathways activated by capsaicin in cancer cells. Capsaicin and several related compounds are called capsaicinoids and are produced as secondary.
Capsaicin has been shown to induce apoptosis in many different types of cancer cell lines including pancreatic 19 colonic 24, prostatic 25, liver 26. Oct 26, 2009 effect of capsaicin on proliferation, cell cycle and apoptosis of breast cancer cells. Capsaicin induces cell cycle arrest and apoptosis in human. The researchers found that when cancer cells were treated with either capsaicin or resiniferatoxin, more than half underwent apoptosis cancer cell death. Here, we report that it has a profound antiproliferative effect on prostate cancer cells, inducing the apoptosis of both androgen receptor arpositive lncap and negative pc3, du145 prostate cancer cell lines associated with an increase of p53, p21, and bax. The aim of this work was to investigate whether combination therapy with capsaicin. The conflicting role of capsaicin derived from cell and animal studies. In this study, both gastric cancer and normal epithelial cells were treated with capsaicin and examined for apoptosis by annexin v binding. Our study showed that the capsaicin treatment significantly induced cell apoptosis and led to prominent upregulation of apoptosisrelated proteins. Capsaicin stops heart attacks and destroys cancer cells. Here, we show that capsaicin induces arrest of the cell cycle at g 2 m phase and causes apoptosis of kb cells. Caspase3 activity apoptosis assays showed that the tumors treated with 10. Capsaicin sensitive leukemic cells were possessed of wildtype p53, resulting in the phosphorylation of p53 at the ser15 residue by the treatment of capsaicin.
It can also be used, believe it or not, as a digestive aid as well as to stop hemorrhoids and ulcers. Capsaicininduced apoptosis of fadu human pharyngeal. Cytoplasmic histoneassociated dna fragments were determined using cell death detection kit. The capsaicin induced apoptosis was blocked by the pretreatment with zvadfmk, a broadrange caspase inhibitor, or acdevdcho, a caspase3 inhibitor. The ability of capsaicin to inhibit the proliferation of a panel of erpositive and negative breast cancer. Capsaicin mediates caspases activation and induces. Studies have shown that capsaicin can induce apoptosis in some cancer cells by unknown mechanisms. Therefore, novel therapeutic agents are urgently needed for this disease. Next, we measured the timekinetics of capsaicininduced apoptosis in h69 cells. Our results demonstrate that capsaicin is a potent anticancer agent, induces apoptosis in cancer cells and produces no significant damage to normal pancreatic cells, indicating its potential use as a novel chemotherapeutic agent for. In addition, by inducing apoptosis and autophagy in numerous types of malignant cell lines, studies have revealed that capsaicin has an inhibitory effect on tumor growth, which demonstrates that capsaicin has potential anticancer effects, including in prostate cancer cells, human nasopharyngeal carcinoma, colon adenocarcinoma 14,15 and.
Capsaicin induces apoptosis in human small cell lung. Pdf anticancer effects and mechanisms of capsaicin in chili. Capsaicin cps, a natural active ingredient of green and red peppers, and a ligand of transient receptor potential vanilloid type 1 trpv1, has been showed potential in suppression of tumorigenesis of several. Capsaicin mediates cell cycle arrest and apoptosis in. Capsaicininduced trib3 upregulation promotes apoptosis in cancer cells rongjaan lin,1,2 ijung wu,1,2 joyu hong,3 banghung liu,4 rueiyue liang,4 teinming yuan,5 showmei chuang4,6,7 1college of life sciences, national chung hsing university, 2taichung hospital, ministry of health and welfare, 3bachelor program of biotechnology, college of. These results reveal that capsaicin may be useful for the prevention of cancer cell growth. Capsaicin induces apoptosis of human melanoma cells. Human rcc accounts for 2 to 3 % of all cancers, and approximately 90 % of kidney cancer, with an increasing incidence. As expected, capsaicin treatment markedly inhibited the proliferation of hct116 cells. Capsaicin increases trpv6 expression in human gastric and prostate cancer cells, and this could potentially lead to excessive increase of intracellular calcium, 15, 34, 37 and trigger apoptosis. Therefore, the current study investigated capsaicin induced apoptosis and autophagy in npctw01 cells. Therefore, we examined the viability, cell cycle progression, and factors associated with apoptosis in kb cells treated with capsaicin. Capsaicininduced apoptosis was in association with the elevation of intracellular reactive oxygen species production.
Renal cell carcinoma rcc is one of the tumors most refractory to chemotherapy to date. Small cell lung cancer sclc is characterized by an aggressive clinical course and a dismal survival rate. Combined effects of capsaicin and ha141 in inducing. M blocked the influx of calcium ions in trpv6transfected jurkat cells. Capsaicin, a spicy component of hot pepper, induces apoptosis. Our study indicates that exposure of human kb cancer cells to capsaicin reduces cell viability, induces cell cycle arrest at g2m phase, and. Therefore there is an urgent need for novel therapeutic modalities for this lethal malignancy. The capsaicininduced apoptosis in kb cells is associated with mitochondrial membrane permeabilization and caspase activation. Trpv1 induced apoptosis of colorectal cancer cells by. Capsaicin mediates caspases activation and induces apoptosis.
As the result shown in figure 2a and 2b supplementary figure 1a and 1b, when cell was treated with capsaicin at low concentration 1020. Trpv6 mediates capsaicininduced apoptosis in gastric. Thus, the effects of capsaicin on various cell types need to be explored. Anticancer effects and mechanisms of capsaicin in chili. Capsaicin causes cellcycle arrest and apoptosis in er positive and. Apoptotic effect of capsaicin on colon cancer caco2. Capsaicin cps, a natural active ingredient of green and red peppers, and a ligand of transient receptor potential vanilloid type 1 trpv1, has been showed potential in suppression of tumorigenesis of several cancers. Despite the accumulating studies of antitumor e ects of capsaicin, there have been very few studies on the e ects of capsaicin on oral cancer cell lines. Antitumorigenic effects of capsaicin in colon cancer. The antiproliferative and apoptotic effects of capsaicin on an. Trpv6 mediates capsaicininduced apoptosis in gastric cancer cellsmechanisms behind a possible new hot cancer treatment justine chow, manith norng, jing zhang, jianyuan chai. Capsaicin, renal cell carcinoma, trpv1, caspases, apoptosis, mapk background as the most common type of kidney cancer, renal cell carcinoma rcc is a worldwide public concern.
Mar 15, 2019 our results demonstrate that capsaicin is a potent anticancer agent, induces apoptosis in cancer cells and produces no significant damage to normal pancreatic cells, indicating its potential use as a novel chemotherapeutic agent for pancreatic cancer, said dr. Effects of capsaicin on induction of apoptosis and. Capsaicin induced cell cycle arrest and apoptosis in colon cancer cells. Studies on various cancer cell lines have shown that capsaicin demonstrates chemopreventive properties by causing cellcycle arrest and inducing apoptosis, or by generating reactive oxygen species ros and depolarizing mitochondrial membranes, and through caspase activation. Capsaicinsensitive leukemic cells were possessed of wildtype p53, resulting in the phosphorylation of p53 at the ser15 residue by the treatment of capsaicin. T he underlying mechanism of capsaicin s antitumor. Capsaicin cleanses the blood and contains high amounts of vitamin c and e and magnesium. Capsaicin causes cellcycle arrest and apoptosis in er. Studies on various cancer cell lines have shown that capsaicin demonstrates chemopreventive properties by causing cellcycle arrest and inducing apoptosis, or by generating reactive oxygen species ros and depolarizing mitochondrial membranes, and.
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